تاثیر تزریق درون تخم‌مرغی عصاره گرده کاج بر رشد و تمایز جنسیتی جوجه‌های گوشتی

   One of the problems of the poultry industry is a lack of flocks in broiler farms Although it in the factories of hatchery broiler chickens and turkey, often Sexing chickens to are selling commercial growers, because sex differences in growth rate and feed efficiency, separating males and females to the necessary recurrence needs of uniform and specified market in terms of production carcasses. The current study was conducted to evaluate the effect of in ovo injection of Pine pollen extract on growth and sex differentiation of broiler chicks. A total of 500 Ross (W-308) egg completely randomized with five treatments were assigned to 4 replicate of 25 eggs in each pen. Treatments included: 1-.Non-injected control 2-Sham control (DMSO) 3- injection 50 mg/L of Pine pollen extract 4-injection 100 mg/L of Pine pollen extract 5- injection 150 mg/L of Pine pollen extract. The economic characteristics, hatching percent and the sex differentiation and the relative expression of IGF-I and IGF-II were evaluated. The results of this study showed that Pine pollen extract had no significant effect on hatching percent, economic characteristics and the sex differentiation; but, in ovo injection of Pine pollen extract decreased the relative expression of IGF-I and IGF-II genes. Relative expression of IGF-I gene in breast tissue and IGF-II in the thigh tissue had a dose response to Pine pollen extract. Injection of 150 mg/L of pine pollen extract significantly increased relative gene expression of IGF-I in breast tissue.